Albion Women were knocked out of the cup in the first round after a six-goal thriller at the University of Wolverhampton Performance Hub.
Despite goals from Jade Arber and Trina Greaves, Barnsley progressed to the next round of the competition, with the Baggies now set to compete to keep their crown as the FA Women’s National League Plate holders.
ALBION: Kinnerley, Jewitt, George, Orthodoxou, Elsmore, Baines, Murray (J Davies 80), Greaves, James (Arber 45), K Davies, Gallop (Reid 64).
Substitutes: Stamps.